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bio rad sequencing cell filter paper  (Bio-Rad)


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    Bio-Rad bio rad sequencing cell filter paper
    FIG. 1. Synthetic forked-DNA substrates. The forked-DNA sub- strates, used to measure the helicase activity of 4A9 protein, contained a 33-bp dsDNA region and two noncomplementary ssDNA tails, 59- and 39-tail, of lengths a and b, respectively. The fork junction is indicated by vertical bars in the sequences and in the cartoon. The <t>sequence</t> of the DNA strands in the largest substrate, the (65/27) forked DNA, is shown. The 59-strand of the (65/27) substrate has a ssDNA tail of 65 bases, and the 39-strand has a ssDNA tail of 27 bases. The bases comprising the 33-bp ds region are underlined in the sequence. Substrates containing gapped dsDNA in either the 59- or the 39-tail (a9 or b9, respectively) have a short oligodeoxynucleotide (gray bars) annealed to the end of the appropriate tail, leaving a ss region between the fork junction and the dsDNA end of the respective tail. The star in the cartoon indicates a possible position of the covalently attached fluorescein moiety or the 32P label.
    Bio Rad Sequencing Cell Filter Paper, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 425 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+sequencing+cell+filter+paper/pm09405431-83-13-13?v=Bio-Rad
    Average 94 stars, based on 425 article reviews
    bio rad sequencing cell filter paper - by Bioz Stars, 2026-06
    94/100 stars

    Images

    1) Product Images from "Asymmetric interactions of hexameric bacteriophage T7 DNA helicase with the 5'- and 3'-tails of the forked DNA substrate."

    Article Title: Asymmetric interactions of hexameric bacteriophage T7 DNA helicase with the 5'- and 3'-tails of the forked DNA substrate.

    Journal: The Journal of biological chemistry

    doi: 10.1074/jbc.272.51.32267

    FIG. 1. Synthetic forked-DNA substrates. The forked-DNA sub- strates, used to measure the helicase activity of 4A9 protein, contained a 33-bp dsDNA region and two noncomplementary ssDNA tails, 59- and 39-tail, of lengths a and b, respectively. The fork junction is indicated by vertical bars in the sequences and in the cartoon. The sequence of the DNA strands in the largest substrate, the (65/27) forked DNA, is shown. The 59-strand of the (65/27) substrate has a ssDNA tail of 65 bases, and the 39-strand has a ssDNA tail of 27 bases. The bases comprising the 33-bp ds region are underlined in the sequence. Substrates containing gapped dsDNA in either the 59- or the 39-tail (a9 or b9, respectively) have a short oligodeoxynucleotide (gray bars) annealed to the end of the appropriate tail, leaving a ss region between the fork junction and the dsDNA end of the respective tail. The star in the cartoon indicates a possible position of the covalently attached fluorescein moiety or the 32P label.
    Figure Legend Snippet: FIG. 1. Synthetic forked-DNA substrates. The forked-DNA sub- strates, used to measure the helicase activity of 4A9 protein, contained a 33-bp dsDNA region and two noncomplementary ssDNA tails, 59- and 39-tail, of lengths a and b, respectively. The fork junction is indicated by vertical bars in the sequences and in the cartoon. The sequence of the DNA strands in the largest substrate, the (65/27) forked DNA, is shown. The 59-strand of the (65/27) substrate has a ssDNA tail of 65 bases, and the 39-strand has a ssDNA tail of 27 bases. The bases comprising the 33-bp ds region are underlined in the sequence. Substrates containing gapped dsDNA in either the 59- or the 39-tail (a9 or b9, respectively) have a short oligodeoxynucleotide (gray bars) annealed to the end of the appropriate tail, leaving a ss region between the fork junction and the dsDNA end of the respective tail. The star in the cartoon indicates a possible position of the covalently attached fluorescein moiety or the 32P label.

    Techniques Used: Activity Assay, Sequencing



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    bio rad sequencing cell filter paper  (Bio-Rad)
    94
    Bio-Rad bio rad sequencing cell filter paper
    FIG. 1. Synthetic forked-DNA substrates. The forked-DNA sub- strates, used to measure the helicase activity of 4A9 protein, contained a 33-bp dsDNA region and two noncomplementary ssDNA tails, 59- and 39-tail, of lengths a and b, respectively. The fork junction is indicated by vertical bars in the sequences and in the cartoon. The <t>sequence</t> of the DNA strands in the largest substrate, the (65/27) forked DNA, is shown. The 59-strand of the (65/27) substrate has a ssDNA tail of 65 bases, and the 39-strand has a ssDNA tail of 27 bases. The bases comprising the 33-bp ds region are underlined in the sequence. Substrates containing gapped dsDNA in either the 59- or the 39-tail (a9 or b9, respectively) have a short oligodeoxynucleotide (gray bars) annealed to the end of the appropriate tail, leaving a ss region between the fork junction and the dsDNA end of the respective tail. The star in the cartoon indicates a possible position of the covalently attached fluorescein moiety or the 32P label.
    Bio Rad Sequencing Cell Filter Paper, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+sequencing+cell+filter+paper/pm09405431-83-13-13?v=Bio-Rad
    Average 94 stars, based on 1 article reviews
    bio rad sequencing cell filter paper - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    FIG. 1. Synthetic forked-DNA substrates. The forked-DNA sub- strates, used to measure the helicase activity of 4A9 protein, contained a 33-bp dsDNA region and two noncomplementary ssDNA tails, 59- and 39-tail, of lengths a and b, respectively. The fork junction is indicated by vertical bars in the sequences and in the cartoon. The sequence of the DNA strands in the largest substrate, the (65/27) forked DNA, is shown. The 59-strand of the (65/27) substrate has a ssDNA tail of 65 bases, and the 39-strand has a ssDNA tail of 27 bases. The bases comprising the 33-bp ds region are underlined in the sequence. Substrates containing gapped dsDNA in either the 59- or the 39-tail (a9 or b9, respectively) have a short oligodeoxynucleotide (gray bars) annealed to the end of the appropriate tail, leaving a ss region between the fork junction and the dsDNA end of the respective tail. The star in the cartoon indicates a possible position of the covalently attached fluorescein moiety or the 32P label.

    Journal: The Journal of biological chemistry

    Article Title: Asymmetric interactions of hexameric bacteriophage T7 DNA helicase with the 5'- and 3'-tails of the forked DNA substrate.

    doi: 10.1074/jbc.272.51.32267

    Figure Lengend Snippet: FIG. 1. Synthetic forked-DNA substrates. The forked-DNA sub- strates, used to measure the helicase activity of 4A9 protein, contained a 33-bp dsDNA region and two noncomplementary ssDNA tails, 59- and 39-tail, of lengths a and b, respectively. The fork junction is indicated by vertical bars in the sequences and in the cartoon. The sequence of the DNA strands in the largest substrate, the (65/27) forked DNA, is shown. The 59-strand of the (65/27) substrate has a ssDNA tail of 65 bases, and the 39-strand has a ssDNA tail of 27 bases. The bases comprising the 33-bp ds region are underlined in the sequence. Substrates containing gapped dsDNA in either the 59- or the 39-tail (a9 or b9, respectively) have a short oligodeoxynucleotide (gray bars) annealed to the end of the appropriate tail, leaving a ss region between the fork junction and the dsDNA end of the respective tail. The star in the cartoon indicates a possible position of the covalently attached fluorescein moiety or the 32P label.

    Article Snippet: After electrophoresis, the gels were fixed in 10% (v/v) acetic acid, dried on Bio-Rad sequencing cell filter paper (2 h at 80 °C), exposed to phosphor storage screens (48 h), and quantitated using a PhosphorImager 445 SI (Molecular Dynamics, Inc.).

    Techniques: Activity Assay, Sequencing